Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2494-2496, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-324343
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis.</p><p><b>METHOD</b>A Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm) column was used with acetonitrile-water (1090) and methnol-water(8614) at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin, sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL x min(-1) and the column temperature was 25 degrees C.</p><p><b>RESULT</b>All of the compounds were based--isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0.344 4(r = 0.999 2) , 0.001 1-0.014 0 (r2 = 0. 999 8), 0.001 1-0.013 4 (r2 = 0.999 9) and 0.001 1-0.008 8 mg x mL(-1) (r2 = 0. 999 6), respectively.</p><p><b>CONCLUSION</b>The method is simple and accurate, which can be used for quality control of S. pseudochinesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oleanolic Acid
/
Plants, Medicinal
/
Pyrones
/
Triterpenes
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Plant Stems
/
Plant Roots
/
Plant Leaves
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2007
Type:
Article
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