Expression and identification of type 1 diabetes associated autoantigen IA-2 / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 524-528, 2003.
Article
in English
| WPRIM
| ID: wpr-324398
ABSTRACT
<p><b>OBJECTIVES</b>To obtain prokaryotic expressed IA-2 recombinant protein and to identify its immunological activity.</p><p><b>METHODS</b>The complimentary DNA (cDNA) coding for the intracytoplasmic part of IA-2 (IA-2ic) was amplified from human fetal brain RNA, and was subcloned into the PinPoint Xa-1 T vector to construct recombinant expression plasmid, and was then expressed in E. coli JM109 cells as a fusion protein with a biotinylated peptide sequence at the aminoterminus. The biotinylated fusion protein was then purified by affinity chromatography and was subsequently dialyzed. Finally, its immunogenicity was evaluated by enzyme linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The purified IA-2ic fusion protein resolved on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as a single Coomassie brilliant blue stained band with a molecular weight of 59 kDa and its immunogenicity was confirmed by ELISA.</p><p><b>CONCLUSIONS</b>E. coli expressed IA-2ic fusion protein has immunological activity. It can be used for detection of IA-2 autoantibodies (IA-2A) and for further studies on type 1 diabetes in future.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Autoantigens
/
Recombinant Fusion Proteins
/
Protein Tyrosine Phosphatases
/
DNA, Complementary
/
Diabetes Mellitus, Type 1
/
Allergy and Immunology
/
Escherichia coli
/
Protein Tyrosine Phosphatase, Non-Receptor Type 1
/
Receptor-Like Protein Tyrosine Phosphatases, Class 8
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2003
Type:
Article
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