Antioxidative and anti-endotoxin effects of propofol on endothelial cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 731-735, 2003.
Article
in English
| WPRIM
| ID: wpr-324422
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the antioxidant and anti-endotoxin effects of propofol on endothelial cells and the possible mechanisms.</p><p><b>METHODS</b>Cultured endothelial cells were treated with hydrogen peroxide (H(2)O(2)), propofol + H(2)O(2), lipopolysaccharide (LPS) and propofol + LPS, respectively. Endothelial cell damage was monitored for possible lactic dehydrogenase (LDH) release. The transcription and the protein expression levels of endothelial nitric oxide synthase (eNOS) were measured.</p><p><b>RESULTS</b>LDH release was higher in groups treated with H(2)O(2) or LPS than in the control group. After pretreatment with propofol, the effects induced by H(2)O(2) were attenuated, but propofol did not decrease the LDH release induced by LPS. Both H(2)O(2) and LPS significantly increased the eNOS transcript levels and the increases were significantly attenuated after pretreatment with propofol. Both H(2)O(2) and LPS significantly increased the eNOS protein expression and the increase was attenuated after pretreatment with propofol.</p><p><b>CONCLUSION</b>Propofol could protect endothelial cells against oxidative stress by inhibiting eNOS transcription and protein expression, but could not antagonise endotoxin induced cell injuries.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
In Vitro Techniques
/
Endothelium, Vascular
/
Propofol
/
Lipopolysaccharides
/
Free Radical Scavengers
/
Nitric Oxide Synthase
/
Cell Biology
/
Endotoxins
/
Nitric Oxide Synthase Type III
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2003
Type:
Article
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