Effect of CCH1 and MID1 in calcium influx under alkaline pH and its regulation by Crz1p transcription factor in Candida albicans / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 917-925, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-324486
ABSTRACT
In Candida albicans, adaptation to environmental pH is relevant to its pathogenicity. Calcium signaling pathway involves in many stress responses and often accompany with Ca2+ fluctuation. We constructed CCH1 and MID1 mutant strains and studied their effect on calcium influx and further investigated the regulation by Crz1p transcription factor. We used PCR-directed gene disruption to construct cch1delta/delta and mid1delta/delta null mutant. By using a flow cytometry-based method we monitored the free cytosolic Ca2+ levels under alkaline stress. Moreover, we constructed pPHO89-LacZ plasmids and by beta-Galactosidase assays, we analyzed the changes of LacZ activities after gene disruption. The results showed that alkaline stress induced calcium burst reduced obviously in cch1delta/delta and mid1delta/delta mutant strains, also for LacZ activities, and fully abolished in crz1delta/delta mutant strain. Finally, by realtime PCR, we confirmed the regulation role of Crz1p in CCH1 and MID1 genes but in a calcineurin independent way. Studies on the effect of calcium pathway on response to alkaline stress will provide an important theoretical basis for Candida albicans infection-oriented treatment and new drug targets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Stress, Physiological
/
Transcription Factors
/
Candida albicans
/
Fungal Proteins
/
Calcium Channels
/
Signal Transduction
/
Gene Expression Regulation, Fungal
/
Genetics
/
Hydrogen-Ion Concentration
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS