Expression and characterization of a new class IIa bacteriocin / 生物工程学报
Chinese Journal of Biotechnology
; (12): 976-982, 2011.
Article
in Zh
| WPRIM
| ID: wpr-324513
Responsible library:
WPRO
ABSTRACT
NB-Cl gene is a potential class IIa bacteriocin gene. To obtain its soluble expression, NB-C1 was fused with the green fluorescent protein (GFP) gene and a recombinant expression vector plVEX 2.4d-GFP-NB-C1 was constructed, which was transformed into Escherichia coli BL21(DE3) pLysS. The expressed fusion protein GFP-NB-CI was purified by Ni-NTA affinity chromatography and the bioactivity was examined using Listeria monocytogenes as the indicator bacteria. The results showed that the expressed fusion protein GFP-NB-C1 was soluble and the final concentration of the purified fusion protein was 36.1 mg/L E. coli culture and had the purity above 95%. The antimicrobial assay of GFP-NB-C1 was analyzed and showed its high activity against Listeria monocytogenes.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Bacteriocins
/
Recombinant Fusion Proteins
/
Molecular Sequence Data
/
Chromatography, Affinity
/
Amino Acid Sequence
/
Green Fluorescent Proteins
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Escherichia coli
/
Genetic Vectors
/
Genetics
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article