Preparation and characterization of follwing the national standard anti-Brucella abortus serum, bovine / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 812-816, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-324534
ABSTRACT
To prepare anti-Brucella abortus serum used for calibrate the agglutination test follwing the national standard, 4 anti-Brucella abortus sera were obtained from 4 cows infected with Brucella abortus naturally. By potency testing, the third serum was selected. Sterility, vaccum degree, residual moisture, uniformity and stability of this standard material were tested and proved to meet the national standard. Referring to the international standard, RBT (Rose-Bengal plate agglutination test), SAT (standard tube agglutination) and CFT (complement fixation test) titers of this standard material were measured to be 1160 "+" 12 400 "++" and 1800 "++", which are identical with the collaborative assay results. International unit of the standard material is 4 000 IU/mL.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reference Standards
/
Blood
/
Brucella abortus
/
Brucellosis, Bovine
/
Agglutination Tests
/
Complement Fixation Tests
/
China
/
Diagnosis
/
Allergy and Immunology
/
Immune Sera
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2011
Type:
Article
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