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Determination of two saponins in Tupistra chinensis rhizomes by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2647-2649, 2008.
Article in Chinese | WPRIM | ID: wpr-324833
ABSTRACT
<p><b>OBJECTIVE</b>To develog an HPLC method determination of two new saponins in the dried rhizomes of Tupistra chinensis.</p><p><b>METHOD</b>The analysis was performed on YMC-Pack ODS-AQ (4.6 mm x 250 mm, 5 microm) eluted with of acetonitrile and waterin gradient mode. The conentration of acetonitrile in the mobile phase changes from 10% to 100% within 85 minutes. The detection wavelength was set at 203 nm. The flow rate was 1.00 mL x min(-1) and column temperature was set at 30 degrees C.</p><p><b>RESULT</b>The linear relationships of two authentic saponins were determined within the range from 7.55 microg to 60.40 microg (r=0.9996) and 8.00 microg to 48.00 microg (r=0.9996), respectively. Using the method above, the contents of two saponins were determined as 0.261% and 0.242%, with the recoveries as 98.6% and 98.3% with RSD 1.7% and 1.7%, respectively.</p><p><b>CONCLUSION</b>A convenient and reliable method was developed to determine the content of two saponins in the dried rhizomes of Tupistra chinensis.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Saponins / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Magnoliopsida / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Saponins / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Magnoliopsida / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2008 Type: Article