Development of bone marrow mesenchymal stem cell culture in vitro / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1650-1655, 2012.
Article
in English
| WPRIM
| ID: wpr-324920
ABSTRACT
<p><b>OBJECTIVE</b>To review the in vitro development of bone marrow mesenchymal stem cells culture (BM-MSC).</p><p><b>DATA SOURCES</b>The data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were "bone marrow mesenchymal stem cell" and "cell culture".</p><p><b>STUDY SELECTION</b>Articles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional (2D) vs. 3D.</p><p><b>RESULTS</b>Improving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems.</p><p><b>CONCLUSIONS</b>Expansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bone Marrow Cells
/
Cells, Cultured
/
Cell Culture Techniques
/
Cell Biology
/
Mesenchymal Stem Cells
/
Methods
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2012
Type:
Article
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