Expression and mutations of PIK3CA gene in hepatocellular carcinomas / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2517-2522, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325074
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and mutation of PIK3CA gene in hepatocellular carcinomas (HCC).</p><p><b>METHODS</b>HCC samples and the corresponding adjacent tissues were collected from the surgical patients with pathologically verified diagnosis. The exons 1, 9 and 20 of PIK3CA gene were detected by PCR-SSCP and DNA sequencing. Immnohistochemistry was employed to test the expression of PIK3CA gene in these samples.</p><p><b>RESULTS</b>No mutation was found in exons 1, 9 or 20 of PIK3CA gene in the HCC tissue and the adjacent tissues by PCR-SSCP and DNA sequencing, while abnormal superimposed peaks were found on the sequence map of exon 9 in 25 cases of HCC tissue. Immunohistochemistry showed that expression of PIK3CA was higher in the HCC tissue than in the corresponding adjacent tissue (50.81% vs 14.75%).</p><p><b>CONCLUSION</b>PIK3CA gene mutation may exist in HCC in Guangxi, which can be associated with the development of HCC, but the ratio of hotspot mutations is low.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Exons
/
Sequence Analysis, DNA
/
Carcinoma, Hepatocellular
/
Phosphatidylinositol 3-Kinases
/
Class I Phosphatidylinositol 3-Kinases
/
Genetics
/
Liver Neoplasms
/
Metabolism
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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