Identification and genotyping of oncogenic type of human papillomavirus in paraffin-embedded cervical cancer samples in Guangzhou / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2485-2487, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325084
ABSTRACT
<p><b>OBJECTIVE</b>To investigate human papillomavirus (HPV) infection and genotyping in patients with cervical cancer in Guangzhou in the last 3 decades.</p><p><b>METHODS</b>HPV L1 gene fragment in paraffin-embedded cervical cancer samples was amplified by HPV-specific PCR with consensus primers, and typing of HPV strains was performed on the basis of sequence analysis of the PCR products.</p><p><b>RESULTS</b>The positivity rates of HPV DNA was 26.2% in the 99 cases of cervical cancer. Five HPV genotypes were identified including HPV16, 18, 33, 52 and 58.</p><p><b>CONCLUSION</b>HPV16, 58 and 33 are the most common genotypes of HPV, accounting for over 88.4% in the total infected cases, suggesting that the HPV genotypes closely related to cervical cancer is more centralized in Guangzhou.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papillomaviridae
/
Pathology
/
Virology
/
DNA, Viral
/
Uterine Cervical Neoplasms
/
China
/
Polymerase Chain Reaction
/
Epidemiology
/
Papillomavirus Infections
/
Human papillomavirus 16
Type of study:
Diagnostic study
Limits:
Female
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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