Establishment of mouse model of humoral immune response using rabbit red blood cells as the antigen / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2473-2476, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325087
ABSTRACT
<p><b>OBJECTIVE</b>To establish a mouse model of humoral immune response by immunization with rabbit red blood cells (RRBCs).</p><p><b>METHODS</b>The mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry.</p><p><b>RESULTS</b>The peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone (25 mg/kg) and cyclophosphamide (20 mg/kg) inhibited hemolysin production. Mannatide (4 mg/kg) produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide (20 mg/kg).</p><p><b>CONCLUSION</b>Intraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Immunization
/
Models, Animal
/
Allergy and Immunology
/
Erythrocytes
/
Immunity, Humoral
/
Guinea Pigs
/
Hemolysin Proteins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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