Inhibitory effect of dexamethasone on myeloid differentiation factor 88 and tumor necrosis factor-alpha expressions in mouse peritoneal macrophages / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2404-2409, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325105
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibitory effect of dexamethasone (DEX) on myeloid differentiation factor 88 (MyD88) and tumor necrosis factor-alpha (TNF-alpha) expression in mouse peritoneal macrophages in innate immune response to Penicillium marneffei (PM).</p><p><b>METHODS</b>Mouse peritoneal macrophages were cultured in the presence of heat-inactivated yeast-phase PM with or without DEX, and the protein and mRNA expressions of MyD88 in the macrophages were detected using Western blotting and real-time PCR, respectively. TNF-alpha in the cell culture supernatant was measured with enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>DEX suppressed TNF-alpha production by the macrophages co-cultured with PM. The expressions of MyD88 were up-regulated by PM stimulation, whose effect was inhibited by the application of DEX.</p><p><b>CONCLUSION</b>The inhibitory effect of DEX on PM-induced proinflammatory responses of the macrophage is directly associated with the inhibition of MyD88 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penicillium
/
Pharmacology
/
Dexamethasone
/
Cells, Cultured
/
Tumor Necrosis Factor-alpha
/
Macrophages, Peritoneal
/
Cell Biology
/
Myeloid Differentiation Factor 88
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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