Your browser doesn't support javascript.
loading
Inhibitory effect of dexamethasone on myeloid differentiation factor 88 and tumor necrosis factor-alpha expressions in mouse peritoneal macrophages / 南方医科大学学报
Journal of Southern Medical University ; (12): 2404-2409, 2009.
Article in Chinese | WPRIM | ID: wpr-325105
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibitory effect of dexamethasone (DEX) on myeloid differentiation factor 88 (MyD88) and tumor necrosis factor-alpha (TNF-alpha) expression in mouse peritoneal macrophages in innate immune response to Penicillium marneffei (PM).</p><p><b>METHODS</b>Mouse peritoneal macrophages were cultured in the presence of heat-inactivated yeast-phase PM with or without DEX, and the protein and mRNA expressions of MyD88 in the macrophages were detected using Western blotting and real-time PCR, respectively. TNF-alpha in the cell culture supernatant was measured with enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>DEX suppressed TNF-alpha production by the macrophages co-cultured with PM. The expressions of MyD88 were up-regulated by PM stimulation, whose effect was inhibited by the application of DEX.</p><p><b>CONCLUSION</b>The inhibitory effect of DEX on PM-induced proinflammatory responses of the macrophage is directly associated with the inhibition of MyD88 expression.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Penicillium / Pharmacology / Dexamethasone / Cells, Cultured / Tumor Necrosis Factor-alpha / Macrophages, Peritoneal / Cell Biology / Myeloid Differentiation Factor 88 / Genetics / Metabolism Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2009 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Penicillium / Pharmacology / Dexamethasone / Cells, Cultured / Tumor Necrosis Factor-alpha / Macrophages, Peritoneal / Cell Biology / Myeloid Differentiation Factor 88 / Genetics / Metabolism Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2009 Type: Article