Expression of human triggering receptor expressed on myeloid cells 1 in peripheral blood mononuclear cells of patients with acute obstructive suppurative cholangitis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2179-2181, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325153
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression pattern of human triggering receptor expressed on myeloid cells 1 (TREM-1) mRNA in peripheral blood mononuclear cells and its clinical significance in acute obstructive suppurative cholangitis (AOSC).</p><p><b>METHODS</b>Peripheral blood mononuclear cells were collected from 36 patients with AOSC and 40 healthy adults. TREM-1 mRNA was determined by semi-quantitative RT-PCR, and TREM-1 protein by immunocytochemistry. Enzyme-linked immunosorbent assay (TNF-alpha) was used to detect the level of tumor necrosis factor-alpha (TNF-alpha), and immunoturbidimetry employed to detect C reactive protein.</p><p><b>RESULTS</b>The expression of TREM-1 mRNA relative to beta-actin was 1.007-/+0.252 in patients with AOSC, significantly higher than that in the healthy adults (0.457-/+0.053, P<0.05). The two groups also showed significantly different TREM protein expression (P<0.01). The AOSC patients exhibited significantly higher levels of TNF-alpha and C reactive protein than the healthy adults (P<0.01).</p><p><b>CONCLUSION</b>The expression of human TREM-1 in peripheral blood mononuclear cells is up-regulated obviously in early stage of AOSC, probably suggesting an important role of TREM-1 in the development of AOSC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Leukocytes, Mononuclear
/
Membrane Glycoproteins
/
Receptors, Immunologic
/
Biomarkers
/
Case-Control Studies
/
Cholangitis
/
Acute Disease
Type of study:
Observational study
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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