Construction and functional identification of red fluorescent protein reporter plasmid for human catalase gene / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2149-2151, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325161
ABSTRACT
<p><b>OBJECTIVE</b>To construct a red fluorescent protein reporter gene driven by human catalase gene promoter.</p><p><b>METHODS</b>The red fluorescent protein reporter gene plasmid pDsRed-CATp containing human catalase gene promoter was constructed by gene recombination technique. The plasmid was transiently transfected into NIH/3T3 cells to observe their response to H(2)O(2) stimulation.</p><p><b>RESULTS</b>The plasmid was constructed correctly as verified by double enzyme digestion and sequence analysis. The plasmid was lowly expressed in resting NIH/3T3 cells, but the expression level increased obviously after stimulation by H(2)O(2). CONCLSIONS A red fluorescent protein reporter gene plasmid driven by human catalase gene promoter has been constructed successfully with a sensitive response to H(2)O(2) stimulation. This system provides a convenient tool for the study of the regulatory mechanism of catalase gene expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plasmids
/
Transfection
/
Catalase
/
Gene Expression
/
Promoter Regions, Genetic
/
3T3 Cells
/
Genes, Reporter
/
Genetic Vectors
/
Genetics
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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