Expression of transcription factors T-bet and GATA-3 in peripheral blood mononuclear cells from patients with systemic lupus erythematosus / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2138-2139, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325162
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of transcription factors T-bet and GATA-3 in the pathogenesis of systemic lupus erythematosus (SLE).</p><p><b>METHODS</b>The expression of T-bet and GATA-3 mRNA in the peripheral blood mononuclear cells (PBMCs) of 60 patients with SLE and 20 normal control subjects were detected by reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Compared with the normal controls, T-bet mRNA expression decreased whereas GATA-3 mRNA expression increased significantly in patients with SLE, active SLE and inactive SLE.</p><p><b>CONCLUSIONS</b>The high expression of GATA-3 promotes the immature T cells to differentiate into Th2 cells, induces Th2 cells to secrete the cytokines IL-6 and IL-10, which, along with the low expression of Th1 as a result of T-bet expression inhibition, causes B cell activation to produce a large quantity of autoantibodies, leading finally to multiple organ injuries. Effective regulation of GATA-3 expression may provide a new clue for the treatment of SLE.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
RNA, Messenger
/
Leukocytes, Mononuclear
/
Case-Control Studies
/
Reverse Transcriptase Polymerase Chain Reaction
/
T-Box Domain Proteins
/
GATA3 Transcription Factor
/
Genetics
/
Lupus Erythematosus, Systemic
/
Metabolism
Type of study:
Observational study
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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