Cloning and sequence analysis of the gene encoding NiFe-hydrogenase from Klebsiella pneumoniae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 133-137, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-325405
ABSTRACT
Degenerate PCR primers were designed by multiple alignment of the protein sequences of known structural genes encoding the catalytic subunits of NiFe-hydrogenases obtained from Swiss-Prot Protein Sequence Database through CLUSTAL-W software and compared for conserved sequence motifs. An amplified PCR product 1 kb in size was obtained from the genomic DNA of Klebsiella pneumoniae using a set of degenerate primers, and then inverse PCR technique was used to obtain the full hydrogenase coding region. A predicted secondary structure and 3D structural model were constructed by homology modeling and docking. On the basis of these results, it was inferred that NiFe-hydrogenase from Klebsiella pneumoniae belongs to the membrane-bound H2 evolving hydrogenase group (Ech hydrogenase group).
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Conformation
/
Bacterial Proteins
/
Codon
/
DNA, Bacterial
/
Molecular Sequence Data
/
Models, Molecular
/
Chemistry
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Protein Structure, Tertiary
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article
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