Regeneration of transgenic Lespedeza thunbergii expressing levansucrase gene (Sac B) from Bacillus subtilis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 940-944, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-325446
ABSTRACT
The Bacillus subtilis Sac B gene with the vacuolar targeting signal sequence driven by 35S promotor was transferred into Lespedeza thunbergii by Agrobacterium mediated method. Total 62 Kan-resistant plants were obtained, of which 5 plants were proved to be transgenic plants. The transgenic plants were characterized by PCR amplification, PCR-Southern hybridization and RT-PCR. The physiological assay results showed that the transgenic plants were more tolerant to stress than the controls under the condition of 200mmol/L NaCl and 5% PEG, respectively, and that the content of soluble sugar in trnsgenic plants was significantly higher than that of controls in the period of tests (5-15 days) under salt and PEG stress.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Solubility
/
Stress, Physiological
/
Bacillus subtilis
/
Transformation, Genetic
/
Carbohydrates
/
Sodium Chloride
/
Chemistry
/
Plants, Genetically Modified
/
Transgenes
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
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