Study on vitrification of porcine embryos by open pulled straw method / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 845-849, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-325461
ABSTRACT
291 embryos (Blastocyst/Morula) from 20 donor sows were vitrified by two step method with OPS (open pulled straw) in solution I (TCM199 + 20% FBS + 10% EG + 10% DMSO) for 3min, and solution II (TCM199 + 20% FBS + 20% EG + 20% DMSO + 0.4mol/L SUC) for 1min, stored in liquid nitrogen for 3 months, and transferred into 8 recipient sows after warming, one recipient sow was pregnant and 8 alive piglets were born. This is the first paper to report getting alive piglets by vitrification in China.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Swine
/
Blastocyst
/
Cryopreservation
/
Embryology
/
Embryo Transfer
/
Methods
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
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