Effect of deltamethrin on benzyloxyresorufin O-dealkylatase activity and CYP2B1/2B2 protein level in rat brain / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 422-424, 2002.
Article
in Zh
| WPRIM
| ID: wpr-325490
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the effect of deltamethrin (DM) on benzyloxyresorufin O-dealkylatase (BROD) activity and the expression of CYP2B1/2B2 in rat brain.</p><p><b>METHODS</b>BROD activity was determined by fluorophotometry under the treatment of DM in vivo and vitro. Western-blot analysis was used to detect the expression of CYP2B1/2B2.</p><p><b>RESULTS</b>In vivo, DM could markedly inhibit BROD activity in rat brain microsome after rats had been treated with DM (12.5 mg.kg-1.d-1, i.p.) for 5 days. The inhibitory rate in whole brain, cerebral cortex and cerebellum were 26.7%, 23.8% and 33.3% respectively(P < 0.05). However, in vitro, under the concentration of 2 x 10(-8)-2 x 10(-4) mol/L of DM, there was no obvious change of BROD activity in rat brain. Moreover, Western-blot analysis indicated that DM could significantly reduce the expression of CYP2B1/2B2 in vivo, the inhibitory rate of protein synthesis was 42.6%.</p><p><b>CONCLUSION</b>DM could inhibit BROD activity in rat brain and this effect may be related to the reduction of CYP2B1/2B2 protein synthesis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pyrethrins
/
Steroid Hydroxylases
/
Brain
/
Aryl Hydrocarbon Hydroxylases
/
Blotting, Western
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Cytochrome P-450 CYP2B1
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Enzyme Inhibitors
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Toxicity
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Insecticides
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Nitriles
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2002
Type:
Article