Human papillomavirus type 16 E5 sequence evolution analysis / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 452-454, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325514
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the distribution of HPV 16 variants in Han women patients without Cervical intraepithelial neoplasia in the diagnosis and treatment center for cervical disease, department of Obstetrics and Gynecology in China-Japan friendship hospital with HPV 16 E5 sequence phylogenetic analysis.</p><p><b>METHODS</b>PCR amplification of HPV 16 E5 sequences and sequenced. The association between variations types and different cervical lesions was analyzed.</p><p><b>RESULTS</b>In this research, We first found that variant classification based on HPV 16 E5 DNA sequence (236 bp) alone had high rate of accuracy. In addition, for the first time, our research revealed that single-4075T can distinguished the As variant from all other variants.</p><p><b>CONCLUSION</b>If E5 sequence was used for phylogenetic analysis, it will greatly reduce the experimental costs and improve efficiency and cost-effectiveness. For the first time, our research revealed that single 4075T can distinguished the As variant from all other variants.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Genetic Variation
/
Virology
/
Molecular Sequence Data
/
Cervix Uteri
/
Oncogene Proteins, Viral
/
Classification
/
Evolution, Molecular
/
Papillomavirus Infections
/
Human papillomavirus 16
Limits:
Adult
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Type:
Article
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