Expression of partial S gene of Hantavirus Z10 in Pichia pastoris / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this study is to express partial S gene of Hantavirus Z10.</p><p><b>METHODS</b>The 300 bp S gene of Z10 strain was synthesized by using a successive PCR method for the optimal expression in Pichia pastoris and subcloned into pMD19-T. The SP300 gene was constructed into pPICZaA and sequenced. The recombinant pPICZaA-SP300 and pPICZaA-S300 was transformed into Pichia with LiCI.</p><p><b>RESULTS</b>The recombination Pichia were cultivate, and expressed the SP300 or S300 gene induced in Pichia by methanol.</p><p><b>CONCLUSION</b>The nucleocapsid secreted from the Pichia can be detected by ELISA and WesternBlot.</p>