The approaches for making acute-on-chronic liver failure in rat / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 394-396, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325531
ABSTRACT
<p><b>OBJECTIVE</b>To study the approaches for making acute-on-chronic hepatic failure model in rat.</p><p><b>METHODS</b>SD rats were intraperitoneally injected with 50% CCl4 (Carbon tetrachloride) olive solution every three days for 6 or 10 weeks. Then they were divided into two groups randomly and injected with D-galactosamine (D-gal) at a dose of 2 g/kg, lipopolysaccharide (LPS) at a dose of 100 pg/kg and D-gal at a dose of 0.5 g/kg BW respectively. The levels of serum ALT, AST and TBil were detected,and histopathological changes were observed to evaluate these two models.</p><p><b>RESULTS</b>After injection of 50% CCl4 olive solution intraperitoneally for 6 weeks, liver fibrosis happened in SD rats, 10 weeks, cirrhosis happened. Pelleta necrosis and massive or submassive necrosis was seen administration of two reagents mentioned above.</p><p><b>CONCLUSION</b>By injecting of 50% CCl4 olive solution intraperitoneally, acute-on-chronic liver failure model could be induced by D-gal, LPS/D-gal in rats.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Carbon Tetrachloride
/
Random Allocation
/
Lipopolysaccharides
/
Mortality
/
Rats, Sprague-Dawley
/
Liver Failure, Acute
/
Alanine Transaminase
/
Disease Models, Animal
/
Galactosamine
Type of study:
Controlled clinical trial
/
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Type:
Article
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