Development a method of quantitative assay for enterovirus 71 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 388-390, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325533
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quantitative assay for enterovirus 71, this can be used in detecting the virus content during vaccine development and production.</p><p><b>METHODS</b>We established the method of quantitative assay for EV71 by using double antibody sandwich ELISA. The sensitivity, accuracy,precision and specificity of the method were evaluated.</p><p><b>RESULTS</b>We developed an ELISA method to quantitative assay for EV71. The quantitation limit of the method is 0.23 microg/ml and the quantitation scope of the method is 7.32-0.23 microg/ml, the coefficient correlation is R2 = 0.9976; The method showed good accuracy, precision and specificity. The recovery is between 90%-110% and the variation coefficient is lower than 10%.</p><p><b>CONCLUSION</b>An ELISA method was developed for the quantitative assay of EV71 virus, which can be used for the rapid quantitative determination of EV71 virus during vaccine development and production.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Enzyme-Linked Immunosorbent Assay
/
Cell Line
/
Sensitivity and Specificity
/
Enterovirus
/
Diagnosis
/
Enterovirus Infections
/
Allergy and Immunology
/
Methods
/
Antigens, Viral
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Type:
Article
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