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Study on effect of enzyme linked immunosorbent assay kits of norovirus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 227-228, 2009.
Article in Chinese | WPRIM | ID: wpr-325583
ABSTRACT
<p><b>OBJECTIVE</b>Effects of RIDASCREEN Norovirus (C 1401) 3rd Generation kit (R-biopham AG, darmstadt, Germany) and IDEIA NLV kit (DAKOCytomation., Ely, UK) were compared for detecting human norovirus (HuNV) in fecal sample.</p><p><b>METHODS</b>The performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction (RT-PCR) by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Guang Zhou. Gene sequencing was performed to positive samples tested by RT-PCR to determine genotype compared with standard sequences.</p><p><b>RESULTS</b>RT-PCR is gold standard, RIDASCREEN Norovirus (C 1401) 3rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76%.</p><p><b>CONCLUSION</b>RIDASCREEN Norovirus (C 1401) 3rd Generation kit is more Satisfactory for a preliminary screening.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Reagent Kits, Diagnostic / Virology / Enzyme-Linked Immunosorbent Assay / Chemistry / Disease Outbreaks / Caliciviridae Infections / Norovirus / Diagnosis / Allergy and Immunology / Feces Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Reagent Kits, Diagnostic / Virology / Enzyme-Linked Immunosorbent Assay / Chemistry / Disease Outbreaks / Caliciviridae Infections / Norovirus / Diagnosis / Allergy and Immunology / Feces Type of study: Diagnostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2009 Type: Article