Detection of human cyclin C gene expression in childhood acute lymphocytic leukemia using real-time fluorescence quantitative PCR / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 14-16, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-325645
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between human cyclin C (CCNC) gene and childhood acute lymphocytic leukemia (ALL).</p><p><b>METHODS</b>The total RNA isolated from myeloid tissues of normal children and of children with newly diagnosed ALL and from ALL cell line 6T-CEM was reversely transcribed into cDNA. Real-time fluorescence quantitative PCR method was used to detect CCNC gene expression.</p><p><b>RESULTS</b>CCNC was expressed in myeloid tissues of normal children and of children with newly diagnosed ALL as well as 6T-CEM. The relative expression level of CCNC gene in children with newly diagnosed ALL was significantly lower than in normal controls (2.35 +/- 0.83 vs 13.5 +/- 0.30; P <0.05).</p><p><b>CONCLUSIONS</b>CCNC gene shows lower expression in children with newly diagnosed ALL, suggesting that it may be a tumor suppressing gene in childhood ALL.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymerase Chain Reaction
/
Cyclins
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
Cyclin C
/
Fluorescence
/
Genetics
/
Metabolism
/
Methods
Type of study:
Diagnostic study
Limits:
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2008
Type:
Article
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