Relationship between copper injury and apoptosis and the effect of curcumin on copper-injured BRL cells / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 567-570, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-325669
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between copper-induced apoptosis and reactive oxygen species (ROS) in BRL cells and the effect of curcumin, a plant-derived polyphenol, on copper-injured BRL cells.</p><p><b>METHODS</b>BRL cells were treated with CuSO4 (100 micromol/L) or curcumin + CuSO4. The BRL cells without any treatment were used as controls. Flow cytometry was applied to detect the production of ROS with fluorescent probe DCFH-DA. MTT colorimetry was used to evaluate cell activity. Apoptosis was measured using Hoechst 33258 staining and Annexin V-FITC and propidiumiodide (PI) staining. JNK/SAPK protein level was detected using Western blot.</p><p><b>RESULTS</b>ROS levels (711.70 +/- 68.33 vs 87.22 +/- 7.58) and apoptosis rate (45.08 +/- 1.87% vs 8.23 +/- 2.56%) of BRL cells reached to a peak after 6 hrs of CuSO4 treatment, which were significantly higher than controls (P < 0.01). JNK/SAPK levels increased significantly after 6 hrs of CuSO4 treatment and peaked at 24 hrs of CuSO4 treatment compared with controls (P < 0.01). Curcumin pretreatment decreased significantly ROS and JNK/SAPK levels as well as the apoptosis rate when compared with the CuSO4-treated alone group (P < 0.01).</p><p><b>CONCLUSIONS</b>Copper may induce apoptosis of BRL cells. ROS participated in apoptosis induced by copper. Curcumin produced protections on copper-injured BRL cells possibly by anti-oxidation and inhibition of p-JNK expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Phosphorylation
/
Cell Line
/
Reactive Oxygen Species
/
Apoptosis
/
Copper
/
Curcumin
/
JNK Mitogen-Activated Protein Kinases
/
Toxicity
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2007
Type:
Article
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