Comparison of the mechanisms of intralesional steroid, interferon or verapamil injection in the treatment of proliferative scars / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 37-40, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-325803
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of intralesional steroid, interferon alpha-2b or verapamil injection on proliferation, apoptosis and TGF-beta1 expression in keloid and hypertrophic scar in vivo.</p><p><b>METHODS</b>6 patients with keloids and 6 patients with hypertrophic scar were treated with intralesional injection of triamcinolone acetonide (40 mg/ml) or IFN alpha-2b (15 x 10(5) U/ml) or verapamil (2.5 mg/ml). Samples were collected on the 7th day after intralesional injection. Samples of untreated keloid and hypertrophic scar and normal skin were used as control. Expression of PCNA and TGF-beta1 was detected in situ by immunohistochemical staining, and apoptosis was detected in situ by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL).</p><p><b>RESULTS</b>1) Triamcinolone acetonide could prohibit proliferative scars through inhibiting cell proliferation and TGF-beta1 expression, as well as inducing apoptosis. 2) IFN alpha-2b could prohibit proliferative scars through inhibiting cell proliferation and TGF-beta1 expression, but not inducing apoptosis; 3) Verapamil could also prohibit proliferative scars through inhibiting proliferation and TGF-beta1 expression in fibroblasts, as well as inducing apoptosis. While the effect of inducing apoptosis was stronger than that of triamcinolone acetonide, the effect of inhibiting TGF-beta1 expression was weaker than those of triamcinolone acetonide and IFN alpha-2b.</p><p><b>CONCLUSIONS</b>Although intraleional injection of steroid, interferon alpha-2b or verapamil were all effective in the treatment of keloid and hypertrophic scar, their mechanisms are not similar.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Steroids
/
Recombinant Proteins
/
Verapamil
/
Interferon-alpha
/
Cicatrix, Hypertrophic
/
Apoptosis
/
Proliferating Cell Nuclear Antigen
/
Therapeutic Uses
/
Drug Therapy
/
Transforming Growth Factor beta1
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2009
Type:
Article
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