Experimental studies of VEGF-C gene for the treatment of chronic obstructive lymphedema in mouse tail model / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 207-211, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-325872
ABSTRACT
<p><b>OBJECTIVE</b>To study the therapeutic effect of vascular endothelial growth factor C (VEGF-C) gene for chronic obstructive lymphedema in mouse tail model which may provide a new treatment for lymphedema.</p><p><b>METHODS</b>RT-PCR and immunoabsorption were applied to detect VEGF-C gene expression in fibroblasts and secretion of VEGF-C protein in COS7 cells respectively after pCDNA3.1 (+) VEGF-C transfection. A mouse tail model of chronic obstructive lymphedema was created. Then the pcDNA3.1-VEGF-C plasmid was injected into the tail. The effect of modulating lymphangiogenesis was observed.</p><p><b>RESULTS</b>Compared with control group, overexpression of VEGF-C enhanced lymphangiogenesis in vivo and mouse tail skin suffering chronic obstructive lymphedema was improved by VEGF-C gene significantly.</p><p><b>CONCLUSIONS</b>VEGF-C can improve the lymphedema through enhancing lymphangiogenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Therapeutics
/
Transfection
/
Genetic Therapy
/
Cells, Cultured
/
Chlorocebus aethiops
/
Gene Transfer Techniques
/
COS Cells
/
Vascular Endothelial Growth Factor C
/
Disease Models, Animal
Type of study:
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2008
Type:
Article
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