Rapid determination of 28 pesticides and tetramine in whole blood by online gel permeation chromatography coupled with gas chromatography-mass spectrometry / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 225-228, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-326026
ABSTRACT
<p><b>OBJECTIVE</b>To study the influences of carbon disulfide (CS2) exposureon fatty acid metabolism in apolipoprotein E (ApoE) knockout mice and C57BL/6J mice.</p><p><b>METHODS</b>Twenty-four male ApoE knockout mice were randomly and equally divided into four groups a CS2-exposed normal diet group, a CS2-unexposed normal diet group, a CS2-exposed high-fat diet group, and a CS2-unexposed high-fat diet group. Twenty-four C57BL/6J male mice were divided into four groups in the same way. The CS2-exposed groups were exposed to CS2 (1 g/m³) by static inhalation for 5 hours a day, 5 days a week. After two weeks, the whole blood of mice was collected. Methyl ester derivatization of fatty acids was performed using an acid-catalyzed method. Fatty acid contents before and after exposure were compared by gas chromatography-mass spectroscopy.</p><p><b>RESULTS</b>There were significant differences in fatty acid contents of mice between the four groups. For the C57BL/6J mice, the arachidic acid contents in the CS2-exposed high-fat diet group were significantly lower than those in the CS2-unexposed high-fat diet group (P = 0.0450). For the ApoE knockout mice, the arachidonic acid contents in the CS2-exposed normal diet group were significantly lower than those in the CS2-unexposed control diet group (P = 0.0452). For the ApoE knockout mice, the γ-linolenic acid contents in the CS2-exposed high-fat diet group were significantly higher than those in the unexposed high-fat diet group (P = 0.0447).</p><p><b>CONCLUSION</b>Exposure to CS2 can induce fatty acid metabolism disorder in mice, indicating that CS2 may increase the risk of atherosclerosis and other cardiovascular diseases.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Apolipoproteins E
/
Carbon Disulfide
/
Risk Factors
/
Mice, Knockout
/
Atherosclerosis
/
Lipid Metabolism
/
Toxicity
/
Diet, High-Fat
/
Gas Chromatography-Mass Spectrometry
/
Mice, Inbred C57BL
Type of study:
Etiology study
/
Risk factors
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2015
Type:
Article
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