Comparison of two nonalcoholic hepatocellular steatosis models / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 297-299, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-326379
ABSTRACT
<p><b>OBJECTIVE</b>To compare two models of nonalcoholic hepatocellular steatosis.</p><p><b>METHODS</b>HL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50% fetal bovine serum to induce fat-overloading. Significant fat accumulation was documented by Oil Red O staining , and intracellular triglyceride levels was detected by triglyceride enzymatic assay.</p><p><b>RESULTS</b>The results showed that both 0.5 mmol/ml oleate acid and 50% FBS were able to induce nonalcoholic hepatocellular steatosis.</p><p><b>CONCLUSION</b>A nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Cell Line
/
Hepatocytes
/
Fatty Liver
/
Models, Biological
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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