No correlation between the sensitivity to 5-aza-dC and the global DNA methylation level in hepatocellular carcinoma cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 284-287, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-326382
ABSTRACT
<p><b>OBJECTIVE</b>To compare the sensitivity of different hepatocellular carcinoma (HCC) cell lines (HepG2, QGY7701, HepG2.2.15) and the normal liver cell line L02 to 5-aza-dC, an DNA methyltransferase inhibitor, and to explore the relationship between global DNA methylation level and the sensitivity to 5-aza-dC.</p><p><b>METHODS</b>HepG2, QGY7701, HepG2.2.15 and L02 cells were treated with 5-aza-dC at different concentration, cell proliferation was measured by MTT method, cell apoptosis was detected by measuring caspase 3 activity and cellular DNA fragmentation ELISA.</p><p><b>RESULTS</b>Compared to HepG2 and QGY7701 cells, HepG2.2.15 were less sensitive to the treatment of 5-aza-dC; the normal liver cell line L02 was less sensitive to 5-aza-dC than the HCC cell lines.</p><p><b>CONCLUSIONS</b>The sensitivity to 5-aza-dC of HCC cell lines and normal liver cells is not correlated with the global DNA methylation level.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Azacitidine
/
DNA Modification Methylases
/
Carcinoma, Hepatocellular
/
DNA Methylation
/
Caspase 3
/
Hep G2 Cells
/
Metabolism
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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