Clinical significance of large-scale screening of A1555G mutation of mitochondria DNA for neonates / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 414-416, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-326921
ABSTRACT
<p><b>OBJECTIVE</b>To explore the necessity of large-scale screening of mitochondria DNA (mtDNA) A1555G mutation for prevention of aminoglycoside antibiotic induced deafness in newborns.</p><p><b>METHODS</b>One thousand blood filter samples were collected from neonates born in July 2008 in Shenzhen. DNA was extracted with Chelex-100 Resin and amplified by PCR. The mtDNA A1555G mutation was determined by denaturing high-performance liquid chromatography(DHPLC) for PCR products. The positive frequency was calculated.</p><p><b>RESULTS</b>The mitochondrial DNA A1555G mutation was detected in 2 cases of 1000 neonates. The frequency of mutation was 0.2%.</p><p><b>CONCLUSION</b>There is a high frequency of mtDNA A1555G mutation in neonates, the large-scale screening of mtDNAA1555G mutation in newborns might detect the individuals sensitive to aminoglycoside antibiotic, which is helpful to guide a rational medication for newborns and the maternal relatives at high-risk. Furthermore, it might be useful to prevent aminoglycoside antibiotic induced deafness.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Mitochondrial
/
DNA Mutational Analysis
/
Base Sequence
/
Polymerase Chain Reaction
/
Genetics
/
Methods
Type of study:
Diagnostic study
/
Screening study
Limits:
Female
/
Humans
/
Male
/
Infant, Newborn
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2011
Type:
Article
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