Chromatin immunoprecipitation and its preliminary application for gene regulation / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 52-55, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-326984
ABSTRACT
<p><b>OBJECTIVE</b>To verify the binding of p53 to p21WAF1/CIP1 gene promoter and detect its binding to hsp90 beta gene promoter in vivo.</p><p><b>METHODS</b>Chromatin immunoprecipitation and PCR analysis were used to measure specific gene regulation sequence and Western blot analysis to investigate p53 protein.</p><p><b>RESULTS</b>The p53 binding sequences on the promoters of p21WAF1/CIP1 and hsp90 beta gene were found in the p53 antibody immunoprecipitated DNA fragments and p53 was detected in the immunoprecipitated samples.</p><p><b>CONCLUSIONS</b>p53 binds to promoters of p21WAF1/CIP1 and hsp90 beta gene in vivo, and regulates the expression of the two genes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Protein Binding
/
Transcription, Genetic
/
Binding Sites
/
Precipitin Tests
/
Chromatin
/
Gene Expression Regulation
/
Polymerase Chain Reaction
/
Tumor Suppressor Protein p53
/
Promoter Regions, Genetic
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article
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