Protective immune mechanisms induced by cellular vaccine made of the erythrocytic Plasmodium yoelii / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 47-51, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-326985
ABSTRACT
<p><b>OBJECTIVE</b>To explore the characteristics of protective immunity against Plasmodium yoelii (P.y.) infection by asexual blood-stages cellular vaccine.</p><p><b>METHODS</b>The particulate vaccines were constructed by saponin or double-distilled-water lysed parasitic red blood cells and inoculated into BALB/c mice by intraperitoneal injection (i.p.). Each group was challenged by the lethal erythrocytic P.y. parasites, and then their parasitemia and survival rates were detected. Expressions of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) were detected by RT-PCR. ELISA showed the serum antibodies against the malaria challenge and their-subclasses. Special membrane protein was recognized by immunofluorescence assay.</p><p><b>RESULTS</b>The vaccination with saponified erythrocytic parasites protected the immunized mice against P.y. challenge, while double-distilled-water lysed vaccine did not (P < 0.01). This protection was characterized by the increase of both IFN-gamma/IgG2a and IL-4/IgG1. Meanwhile, MHC class I alpha chain molecule was recognized on the membrane of infected-erthythrocyte.</p><p><b>CONCLUSION</b>Saponified P.y. asexual blood-stage cellular vaccine has a significantly high protective immunity against this lethal P.y. malaria, and the immunity may be associated with the expression levels of IgG2a and IFN-gamma. MHC class I alpha chain on infected erythrocytes may play an important role in the successful immunization.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Protozoan
/
Plasmodium yoelii
/
Histocompatibility Antigens Class I
/
Random Allocation
/
Vaccination
/
Malaria Vaccines
/
Allergy and Immunology
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article
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