Crosslinking bovine pericardial bioprosthetic heart valves by phthalocyanine sensitized photooxidation reaction / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To crosslink bovine pericardial valves by phthalocyanine sensitized photooxidation reaction.</p><p><b>METHODS</b>Fresh bovine pericardial tissues were immersed in PBS solution containing 0.01% cupreous sulfonatophthalocyanine and exposed to a broad wavelength light source for 20-120 h. Using sodium dodecyl sulfate-polyarylamide gel electrophoresis (SDS-PAGE) to analyze the cross-linking extent of pericardial tissue. With the help of attenuated total reflection-fourier transform infrared (ATR-FTIR), explained the change of protein structure after photooxidation reaction. Differential scanning calorimetry (DSC) was used to measure the change of shrinkage temperature. Tissue water content experiment method was also used.</p><p><b>RESULTS</b>Photooxidized tissue was resistant to extraction when it was compared with untreated tissue and had similar crosslinking extent over glutaraldehyde-treated tissue. The three dimension structure of collagen were changed but not seriously destroyed and the model of photooxidation reaction was validated. Phthalocyanine-treated tissue was much more flexible than glutaraldehyde-treated tissue.</p><p><b>CONCLUSION</b>Bovine pericardial tissue can be successfully crosslinked by phthalocyanine-mediated photooxidation reaction.</p>