Effect of tetramethylpyrazine on learning, memory and cholinergic system in D-galactose-lesioned mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 553-556, 2003.
Article
in Zh
| WPRIM
| ID: wpr-327038
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of tetramethylpyrazine on learning, memory, and cholinergic system in D-galactose-lesioned mice.</p><p><b>METHODS</b>C57BL/6J mice were given subcutaneous injection of 2% D-galactose for 40 days (100 mg.kg-1.d-1). Normal saline, tetramethylpyrazine (TMP) and Huperzine A (HupA) were given respectively by intragastric administration in different study groups from the third week on. Learning and memory ability were tested by Morris water maze for 5 days at the sixth week. Acetylcholinesterase (AchE) activity, the binding sites (Bmax) and the affinity (KD) of M-cholinergic receptor were determined.</p><p><b>RESULTS</b>The learning and memory dysfunction, with lowered AchE activity and M-cholinergic receptor binding sites were found in the model group as compared with the normal control group. The tetramethylpyrazine, especially at the dose of 100 mg.kg-1.d-1, could markedly attenuate cognitive dysfunction, while elevate the lowered AchE activity (P < 0.05) and M-cholinergic receptor binding sites (P < 0.005) in the cerebral cortex of mice treated with D-galactose.</p><p><b>CONCLUSIONS</b>The tetramethylpyrazine can significantly improve central cholinergic system function, and thus enhance the learning and memory ability in D-galactose-lesioned mice.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
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Acetylcholinesterase
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Pyrazines
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Avoidance Learning
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Receptors, Cholinergic
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Cognition
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Maze Learning
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Receptor, Muscarinic M1
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Galactose
Limits:
Animals
Language:
Zh
Journal:
Acta Academiae Medicinae Sinicae
Year:
2003
Type:
Article