Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 504-507, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-327050
ABSTRACT
<p><b>OBJECTIVE</b>To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.</p><p><b>METHODS</b>According to the published SARS coronavirus genome sequences, the full length cDNA of N protein from SARS coronavirus PUMC2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector. The recombinant N protein was expressed in E. coli BL21 (DE3), and purified by Ni(2+)-NTA.</p><p><b>RESULTS</b>Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC2 strain was obtained.</p><p><b>CONCLUSIONS</b>The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
DNA, Viral
/
RNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Amino Acid Sequence
/
Genome, Viral
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
DNA, Complementary
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2003
Type:
Article
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