cDNAs cloning of SARS-CoV PUMC2 viral genome / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 499-503, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-327051
ABSTRACT
<p><b>OBJECTIVE</b>To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain.</p><p><b>METHODS</b>Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced.</p><p><b>RESULTS</b>The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained.</p><p><b>CONCLUSIONS</b>These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Viral Proteins
/
DNA, Viral
/
RNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Amino Acid Sequence
/
Genome, Viral
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
DNA, Complementary
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2003
Type:
Article
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