Your browser doesn't support javascript.
loading
cDNAs cloning of SARS-CoV PUMC2 viral genome / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 499-503, 2003.
Article in Chinese | WPRIM | ID: wpr-327051
ABSTRACT
<p><b>OBJECTIVE</b>To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain.</p><p><b>METHODS</b>Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced.</p><p><b>RESULTS</b>The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained.</p><p><b>CONCLUSIONS</b>These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Viral Proteins / DNA, Viral / RNA, Viral / Molecular Sequence Data / Base Sequence / Amino Acid Sequence / Genome, Viral / Cloning, Molecular / Sequence Analysis, DNA / DNA, Complementary Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2003 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Viral Proteins / DNA, Viral / RNA, Viral / Molecular Sequence Data / Base Sequence / Amino Acid Sequence / Genome, Viral / Cloning, Molecular / Sequence Analysis, DNA / DNA, Complementary Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2003 Type: Article