Selective apoptosis in HeLa cells induced by sodium butyrate and its mechanism / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 401-405, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-327072
ABSTRACT
<p><b>OBJECTIVE</b>To investigate apoptosis induced by sodium butyrate in cervix cancer cell line HeLa and primary human embryo lung fibroblasts and its mechanism.</p><p><b>METHODS</b>Cell apoptosis was assessed by morphology, cell viability, DNA fragmentation, the percentage of sub-G1 cells and phosphatidylserine (PS) externalization. The effects of sodium butyrate on transcription of Bax and Bcl-2 was analyzed by RT-PCR.</p><p><b>RESULTS</b>Sodium butyrate inhibited proliferation in a time and dose-dependant manner. The inhibition of proliferation in HeLa cells was more significant than that in primary human embryo lung fibroblasts. DNA fragmentation, sub-G1 peak and AnnexinV/PI by flow cytometry showed very high apoptosis rates in HeLa cells 72 hours after treated with sodium butyrate, while pretty low in primary human embryo lung fibroblasts. RT-PCR showed sodium butyrate had little effects on transcription of Bax and Bcl-2 in HeLa cells.</p><p><b>CONCLUSION</b>Sodium butyrate can induce apoptosis in HeLa cells without changing the expression of Bax and Bcl-2. Sodium butyrate comparatively has little effects on fibroblasts.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Butyrates
/
HeLa Cells
/
Cell Division
/
Proto-Oncogene Proteins
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Reverse Transcriptase Polymerase Chain Reaction
/
Bcl-2-Associated X Protein
/
DNA Fragmentation
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2003
Type:
Article
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