Collagen synthesis and expression of connective tissue growth factor in the cultured fibroblasts of human hypertrophic scar / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 124-127, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-327291
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar.</p><p><b>METHODS</b>Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods.</p><p><b>RESULTS</b>Compared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01).</p><p><b>CONCLUSION</b>Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Immunohistochemistry
/
Gene Expression
/
Cells, Cultured
/
Collagen
/
Cicatrix, Hypertrophic
/
Immediate-Early Proteins
/
Reverse Transcriptase Polymerase Chain Reaction
/
Intercellular Signaling Peptides and Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2004
Type:
Article
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