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Protective effect of rosmarinic acid on hypoxia/reoxygenation injury in cardiomyocytes / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1897-1901, 2014.
Article in Chinese | WPRIM | ID: wpr-327900
ABSTRACT
<p><b>OBJECTIVE</b>To study the protective effect of rosmarinic acid (Ros A) on the primary cardiomyocyte hypoxia/reoxygenation (H/R) injury.</p><p><b>METHOD</b>Primary cardiomyocytes of rats were cultured in vitro to establish the H/R injury of cardiomyocytes and observe the changes in the cell viability and LDH leakage. The changes in ATP content and ROS in cardiomyocytes were measured by using chemiluminescence and fluorescent probe technique. The effects of rosmarinic acid on the apoptosis of cardiomyocytes, cleaved-caspase 3, Akt and p-Akt protein expression were further detected by flow cytometry and western blot analysis.</p><p><b>RESULT</b>According to the experimental results, Ros A at doses of 25, 50, 100 mg x L(-1) could inhibit the decrease in H/R-induced cell viability, LDH leakage and excessive ROS generation, and maintain the ATP level in cells. Ros A at doses of 50, 100 mg x L(-1) could remarkably inhibit the H/R-induced cardiomyocyte apoptosis and down-regulate the expression of cleaved caspase-3. Moreover, Ros A at doses of 100 mg x L(-1) could significantly up-regulate the expression of p-Akt.</p><p><b>CONCLUSION</b>Ros A has the significant effect in resisting the cardiomyocyte H/R injury, improve cardiomyocyte energy metabolism and reduce cell apoptosis, which is related to the activation of Akt pathway.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Pharmacology / Plant Extracts / Cell Hypoxia / Cell Survival / Cells, Cultured / Adenosine Triphosphate / Chemistry / Cinnamates / Reactive Oxygen Species Limits: Animals / Humans / Male Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Pharmacology / Plant Extracts / Cell Hypoxia / Cell Survival / Cells, Cultured / Adenosine Triphosphate / Chemistry / Cinnamates / Reactive Oxygen Species Limits: Animals / Humans / Male Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article