Isolation and analysis of a high expression promoter in rice / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 836-840, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-327938
ABSTRACT
The expression of plant gene is controlled by its promoter. The isolation and the function analysis of promoter are important for studying the genetic engineering and the regulation expression of plant genes. In this paper, we cloned a promoter, 0s252, which was predicted to be highly expressed in the stem of rice from the EST database. After the construction of the Os252GUS expression vector, it was transformed into rice. The integration of transgenes into transgenic rice genome was confirmed through PCR analysis. X-Gluc staining showed that Os252 can promote GUS gene expression in leaf, stem and matured seed. GUS enzyme activities driven by Os252 promoter in leaf and seed are about 190% and 250% of that driven by the 35S promoter. Thus, the Os252 promoter can be applied for rice genetic engineering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Proteins
/
Oryza
/
Promoter Regions, Genetic
/
Plants, Genetically Modified
/
Gene Expression Regulation, Plant
/
Genetics
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS