An efficient tool for the construction of multiple-cistronic vectors: FMDV 2A / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 765-769, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-327950
ABSTRACT
Recently, cancer therapy with mutiple genes has been attached with great attention. However, at present there is no efficient tool to construct multiple-cistrons. The large sizes and the imbalance in expression of most traditional tools, such as ribosome entry sites (IRESes),greatly block their wide employment in the construction of multiple cistronic gene therapy vectors. The self-cleaving peptide 2A from foot-and-mouth disease virus (FMDV) has a very small size, and more importantly, high cleavage activity in artifical bicistron, which bring new hope for mutiple genes therapy stategy. In this article, the characteristics and cleavage activities of FMDV 2A will be elucidated,and we further outline its applications in cancer gene therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Transcription Factors
/
DNA, Recombinant
/
Molecular Sequence Data
/
Genetic Therapy
/
RNA Splicing
/
Amino Acid Sequence
/
Promoter Regions, Genetic
/
Artificial Gene Fusion
/
Foot-and-Mouth Disease Virus
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS