Preparation and performance of Chitosan-oligosaccharides/DNA complex nanoparticles / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 741-745, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-327954
ABSTRACT
Comparing to Chitosan, Chitosan-oligosaccharides have several special functions, such as water-soluble, antitumor activity, immunostimulating effects, and antimicrobial activity. The chitosan-oligosaccharide, the molecular weight of which was about 5000, was used as research model. According to the agarose gel electrophoresis and UV spectrophotometer it was proved that electrostatic interaction was playing a very important role in the formation process of chitosan-oligosaccharide/DNA complex. The potential of adsorbing DNA on chitosan-oligosaccharide was analyzed by gel electrophoresis and UV spectrophotometer, and it was indicated that chitosan-oligosaccharide can improve the storage and structure stability of DNA. To check its protection ability to DNA by DNase I digestive experiment, the result showed that chitosan-oligosaccharide could load with plasmid effectively and protect DNA from being digested by DNase I. It was proved that chitosan-oligosacchide was safe and effective for gene delivery and will have a very good future in the field of gene therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligosaccharides
/
DNA
/
Chemistry
/
Gene Transfer Techniques
/
Chitosan
/
Nanoparticles
/
Genetic Vectors
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article
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