PTD-hEGF fusion protein--gene expression and function analysis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 589-592, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-327982
ABSTRACT
To produce membrane-permeable human epidermal growth factor (hEGF), a pPTD-hEGF prokaryocyte expression vector was constructed and transformed into E. coli BL 21 (DE3). The PTD-hEGF fusion protein was induced by 0.3 mmol/L of IPTG expressed in the form of inclusion body with an yield of 40% of the total protein in the cells, and then purified by Ni2+ -NTA affinity chromatography. The SDS-PAGE analysis showed a single fusion protein band with a molecular weight of 16 kD. The amino acid sequence was checked by MALDI-TOF-MS analysis. The genetic engineering PTD-hEGF fusion protein obviously promoted the proliferation and growth of the HEK-293 cells in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Transduction, Genetic
/
Recombinant Fusion Proteins
/
Molecular Sequence Data
/
Cell Line
/
Amino Acid Sequence
/
Cell Proliferation
/
Epidermal Growth Factor
/
Escherichia coli
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article
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