Extraction, isolation and purification for ginkgolide B / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1961-1964, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-328051
ABSTRACT
<p><b>OBJECTIVE</b>To establish a simple extraction, isolation and purification method for ginkgolide B from ginkgo leaf.</p><p><b>METHOD</b>The optimum conditions of extraction, isolation and purification were studied by taking the transfer rate of ginkgolide B as index.</p><p><b>RESULT</b>Ginkgo leaf was extracted with 70% ethanol for three times, the extracts were concentrated to remove ethanol and diluted by water till the crude drug density reached 0.1 g x mL(-1). The dilution was adsorbed with HPD-450 macroporous resin. The impurities were eluted with 20% ethanol and ginkgolide B was eluted with 80% ethanol. Then the 80% ethanol eluant was concentrated and crystallized. Finally the crude crystals were recrystallized with isopropanol. The purity of the ginkgolide B recrystallization was 95%.</p><p><b>CONCLUSION</b>The process was stable and easy to operate, which was suited to industrialized production.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Plant Leaves
/
Ginkgo biloba
/
Ginkgolides
/
Chemical Fractionation
/
Lactones
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS