Study on antioxidant effect of Herba Lycopi and screening of active fractions / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To Study the antioxidant activity of Herba Lycopi, and screen the main active fractions.</p><p><b>METHOD</b>The different polar fractions were isolated from the ethanol extract of Herba Lycopi. The scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation of the fractions were studied in vitro by high-throughput screening technique.</p><p><b>RESULT</b>The ethanol extract, ethyl acetate fraction (LLE-A. E, MCCM2921) and n-buthanol fraction (LLE-A. B, MCCM2922) of Herba Lycopi showed the scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation.</p><p><b>CONCLUSION</b>The ethanol extract and different polar fractions showed antioxidant capacity in vitro. That may be explain that the effect of activating blood and dissolving stasis and anti-fibrosis of Herba Lycopi in traditional application. The results may be helpful for further development of Herba Lycopi as a low-toxic antioxidant drug.</p>