Everted intestinal sac method for quick finding absorption ingredients of Wuzhuyu decoction / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1399-1404, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-328112
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for quick finding the absorption ingredients of Wuzhuyu decoction in order to select the index to control its quality.</p><p><b>METHOD</b>The absorption of three concentration of Wuzhuyu decotion was investigated with the in vitro-everted intestinal sac model. The intestinal bag fluid of jejunum and ileum were collected in different time and the eight ingredients, which were evodiamine (Ev), rutaecarpine (Ru), limonin (Li), ginsenoside-Rb1, -Rg1, -Re (Rb1, Rg1, Re), isorhamnetin-3-O-beta-D-glucosyl(6''-->1'")-alpha-L-rhamnoside (Irs)and 6-gingerol (6-Gi), were detected by HPLC as the represent constituents in samples.</p><p><b>RESULT</b>Eight ingredients except Ru in samples could be detected, but Ev could not be detected in high concentration samples. The ratios between absorption ingredients were different from in Wuzhuyu decotion.</p><p><b>CONCLUSION</b>The in vitro-everted intestinal sac canc absorb the ingredients of Wuzhuyu decotion selectivity. Compare with the ileum, the jejunum can provide the more absorption information and faster, the best test time is 60-90 min.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Pharmacokinetics
/
Chemistry
/
Rats, Wistar
/
Extracellular Fluid
/
Drug Evaluation, Preclinical
/
Ileum
/
Intestinal Absorption
/
Jejunum
/
Methods
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
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