Regulation of tissue factor expression by anti-oncogene PTEN in human neuroblastoma cell line / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 363-366, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-328341
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of PTEN gene in the regulation of tissue factor (TF) expression in human neuroblastoma cells.</p><p><b>METHODS</b>Expression of PTEN or TF was determined by Western blotting. Transcription of TF was examined by RT-PCR. PTEN gene expressing vector pCMV-PTEN was transfected with Lipofectamine2000. Phosphorylation of AKT was inhibited by LY294002 and then examined by Western blotting.</p><p><b>RESULTS</b>Human neuroblastoma cell line SK-N-SH was PTEN-positive and expressed low level TF, whereas an other neuroblastoma cell line SK-N-MC was PTEN-negative but expressed high level TF. TF level was downregulated in SK-N-MC cells by enforced expression of PTEN in a dose dependent manner. Inhibition of TF was achieved along with inactivation of AKT. Furthermore treatment with PI3K/AKT inhibitor LY294002 also resulted in decrease of TF expression in a dose-dependent manner.</p><p><b>CONCLUSION</b>Expression of TF is inhibited by PTEN gene via inactivating PI3K/AKT pathway, loss of PTEN might be the explanation of aberrant high-level TF in human neuroblastoma. It may be at least one of the mechanisms by which loss of PTEN expression confers to cancer progression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Thromboplastin
/
Transfection
/
Gene Expression Regulation, Neoplastic
/
Phosphatidylinositol 3-Kinases
/
Cell Line, Tumor
/
PTEN Phosphohydrolase
/
Proto-Oncogene Proteins c-akt
/
Genetic Vectors
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2007
Type:
Article
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