Cerulenin changes apoptosis related genes expression in multiple myeloma cell line U266 / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 239-242, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-328347
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression changes of apoptosis related genes induced by cerulenin in multiple myeloma cell line U266 and explore its molecular mechanism.</p><p><b>METHODS</b>The expression changes of 96 apoptosis related genes were analyzed by superArray cDNA in U266 cells treated with cerulenin (20 microg/ml) for 12 h. Semi-quantitative RT-PCR was used to confirm the representative expression changes genes, Rip2, caspase 9 and TRAF2.</p><p><b>RESULTS</b>After treated with cerulenin for 12 h, 44 apoptosis related genes expression in the U266 cells were changed, among which 41 were over 2 fold increase and 3 over 2 fold decrease. The expression of caspase 9 was increased markedly, indicating that mitochondria pathway played a key role in cerulenin inducing apoptosis and TRAF2 expression change suggested that nuclear factor (NF) participates in cerulenin inducing apoptosis.</p><p><b>CONCLUSION</b>The death acceptor signaling pathway and the death acceptor non-dependence signaling pathway co-regulate cerulenin inducing apoptosis in U266 cells. Mitochondria pathway played the key role and nuclear factor (NF) participates in the apoptosis process.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Signal Transduction
/
Gene Expression Regulation, Neoplastic
/
Cerulenin
/
Apoptosis
/
Gene Expression Profiling
/
Cell Line, Tumor
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2007
Type:
Article
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